Analysis Note

Control3T3/A31 cell lysate, human colon carcinoma tissue

Application

Detect Cyclin E with Anti-Cyclin E Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP, WB & IHC.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 378-396 (GVLTPPHSSKKQSSEQETE) of rat Cyclin E

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-223

Physical form

ImmunoAffinity Purified

PBS containing 0.1% sodium azide and 0.2% gelatin.

Quality

routinely evaluated by immunoblot on RIPA lysates from 3T3/A31 cells

Specificity

Cyclin E

Storage and Stability

stable for 1 year at 4°C from date of shipment

Target description

Mr 52kDa